Tag

Part:BBa_K3815022:Design

Designed by: Alexander Liu   Group: iGEM21_Kyoto   (2021-10-20)


AANDENYAYES. mutant SsrA degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part can be fused to the C-terminus of your proteins of interest to promote their degradation. Note that you do not have to add stop codons because the part includes TAATAA at the 3' end.

Source

Escherichia coli

References